Application

Stable isotope enrichment of proteins is necessary for study by NMR spectroscopy. One way this can be accomplished is using a methylotrophic yeast (Pichia pastoris) expression system for proteins. The use of the patented IsoYeast formulation, which includes isotopically labeled carbon and nitrogen sources as well as several other key chemical components, can improve the expression levels of the targeted proteins versus other defined, rich media. The media are also compatible with inclusion of various amino acids for selective amino acid labeling. No other yeast media currently offer this flexibility.

Each IsoYeast product is a complete system used to prepare dual media - 1 liter of Growth Medium and 250 mL of Expression Medium. The dual media are offered as lyophilized powdered components and need addition of only sterile water to the Growth Medium, and water plus methanol to the Expression Medium.

Packaging

This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.